A thermal cycler/qPCR detection system is a molecular biology instrument that combines precise temperature cycling with real-time fluorescence detection for DNA and RNA analysis. The thermal cycler enables amplification of nucleic acids through polymerase chain reaction (PCR), while the integrated qPCR module monitors fluorescence signals to quantify target sequences during each cycle. This allows accurate measurement of gene expression, mutation detection, and viral or microbial load. Widely used in research, diagnostics, and pharmaceutical development, qPCR systems provide high sensitivity, reproducibility, and throughput for critical molecular biology applications.
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